Method for treating a glycoprotein-related disease

ABSTRACT

A method for treating a glycoprotein-related disease is disclosed, which comprises: administering a first effective amount of phenol red and a second effective amount of an organic arsenic compound to a subject in need thereof.

CROSS REFERENCE TO RELATED APPLICATION

This application claims the benefits of the Taiwan Patent ApplicationSerial Number 104127036, filed on Aug. 19, 2015, the subject matter ofwhich is incorporated herein by reference.

This application also claims the benefits of the Taiwan PatentApplication Serial Number 105106052, filed on Feb. 27, 2016, the subjectmatter of which is incorporated herein by reference.

BACKGROUND

1. Field of the Invention

The present invention relates to a pharmaceutical composition and amethod for treating a glycoprotein-related disease and, moreparticularly, to a pharmaceutical composition and a method for treatinga glycoprotein-related disease with phenol red and an organic arseniccompound.

2. Description of Related Art

Currently, clinical treatment of viral diseases is mainly based onsupportive therapy. Supportive therapy involves fluid therapy andnutrition supplementation to help patients recuperate and to increaseimmunity. It also involves concurrent administration of broad-spectrumantibiotics to prevent secondary infection. However, whether viraldiseases can be cured depends on individual's immunity.

Treatment of snake venom infection requires an initial determination ofthe infection to be hemorrhagic venom infection or neurotoxic venominfection. Administration of anti-snake venom serum then follows toneutralize toxicity and to increase immunity. With concurrentadministration of antibiotics, healing time of wounds with redness andswelling is over 7 days.

Prion infection, such as bovine spongiform encephalopathy, scrapie, andCreutzfeldt-Jakob disease, is caused by abnormal cellular metabolism dueto glycosylation between carbohydrates and proteins in brain cells. Suchabnormal cellular metabolism results in denaturation of proteins toinfective proteins as well as deposition and aggregation ofglycoproteins and amyloids to induce neuropathy. Until now, there isstill no effective drug treatment and prevention for Prion infection.

Toxic protein infection by insects and reptiles, such as mosquitoes,bees, scorpions, spiders, centipedes, ants, and staphylinidae, is causedby injection of toxic proteins into mankind and animals by stinging. Theresult ranges from redness, swelling, allergy, itching, and inflammationof skin to fever. Such insects and reptiles also play roles as media orcarriers of viruses, such as dengue viruses that belong to Flaviviridaeand Flavivirus, West Nile virus, and Zika virus, to infect mankind andanimals.

Cell abnormality includes pathological changes of eye cells, braincells, and cellular metabolism. Cataract, one of the pathologicalchanges of eye cells, is caused by abnormal metabolism and degenerationin the elderly. Such abnormal metabolism and degeneration result inabnormal glycosylation between carbohydrates and proteins in cells ofeye lens. Cataract membrane formation due to deposition and aggregationof protein fibers leads to blurred vision. The current treatment ofcataract includes surgical removal and laser treatment. However, suchtreatments are not completely pain-free and have risks for blindness aswell as high surgery cost. Retinopathy, another pathological change ofeye cells, is caused by glycosylation between carbohydrates and proteinsin photoreceptors cells of fiber membrane and choroidal membrane ofretina. Such glycosylation leads to degeneration and vision loss. Thetreatment of retinopathy generally includes supplementation of vitamin Aand lutein. However, such treatment has no active therapeutic effect.

Alzheimer's disease, one of the pathological changes of brain cells, isalso caused by glycosylation between carbohydrates and proteins in braincells. Such glycosylation results in abnormal cellular metabolism aswell as deposition and aggregation of glycoproteins and amyloids. Theseinduce symptoms of dementia, memory loss, and degeneration of theelderly. Currently, there is no effective prevention and treatment ofAlzheimer's disease. Parkinson's disease is also caused by glycosylationbetween carbohydrates and proteins in neurons of brain. Suchglycosylation results in abnormal metabolism, neurodegeneration, as wellas deposition and aggregation of amyloids and fibrins, leading tochronic neurological symptoms. There is also no prevention and effectivetreatment of Parkinson's disease.

Pancreatitis, a pathological change of cellular metabolism, is caused byglycosylation between carbohydrates and proteins in pancreatic cells.Such glycosylation results in abnormal cellular metabolism,degeneration, as well as deposition and aggregation of glycoproteins andamyloids. These lead to swelling of pancreas, acute and chronicinflammation, and fibrosis of pancreas. Such pancreatitis is oftencategorized as having unknown causes medically. Treatment of suchpancreatitis depends on autologous immunity. There is no effective drugtreatment for such pancreatitis. Kidney inflammation is caused byglycosylation between carbohydrates and proteins in renal cells. Suchglycosylation results in abnormal cellular metabolism and degenerationas well as deposition and aggregation of amyloids. These lead toswelling of kidney, acute and chronic inflammation, and fibrosis ofkidney. Such kidney inflammation is often categorized as having unknowncauses medically. There is no effective drug treatment for such kidneyinflammation. Hepatitis and cholangitis are caused by glycosylationbetween carbohydrates and proteins in hepatic cells. Such glycosylationresults in abnormal cellular metabolism as well as deposition andaggregation of glycoproteins and amyloids. These lead to swelling andinflammation of liver and bile duct as well as fibrosis of liver.Medically, such hepatitis and cholangitis are categorized as acutefulminating hepatitis, acute hepatitis, acute cholangitis, or havingother unknown causes. There is no effective drug treatment for suchhepatitis and cholangitis.

Accordingly, there is a need to develop an effective drug for treatingthe aforesaid diseases.

SUMMARY

The present disclosure provides a method for treating aglycoprotein-related disease, which comprises: administering a firsteffective amount of phenol red and a second effective amount of anorganic arsenic compound to a subject in need thereof.

The present disclosure also provides a pharmaceutical composition fortreating a glycoprotein-related disease in a subject in need thereof,which comprises: a first effective amount of phenol red and a secondeffective amount of an organic arsenic compound.

Furthermore, the present disclosure provides a use of a pharmaceuticalcomposition for manufacturing a medicine for treating aglycoprotein-related disease, wherein the pharmaceutical compositioncomprises: a first effective amount of phenol red and a second effectiveamount of an organic arsenic compound.

Phenol red is known as an acid-base indicator and a diagnosis medicine.The arsenic compound is known as a medicine for treating syphilis, ablood agent, an herbicide and an insecticide.

In the present disclosure, the phenol red and the organic arseniccompound are co-used to inhibit the interaction between carbohydratesand proteins and the glycosylation on proteins in pathogens.

When the phenol red and the organic arsenic compound are co-used, theglycosylation pathway between the carbohydrates (such as sugar, starchand cellulose) and proteins, nucleoside and enzyme of pathogens (such assnake venom, prion, insect venom, reptile venom, virus and abnormalcells) can be inhibited; and the diseases and disorders caused by thepathogens can be inhibited. Hence, the combination of the phenol red andthe organic arsenic compound can be used to treat or prevent virusinfection (including DN and RNA viruses with different structures andshapes), snake venom infection (including hemorrhagic venom infectionand neurotoxic venom infection), prion infection (including bovinespongiform encephalopathy, scrapie, and creutzfeldt-Jakob disease),insect venom infection (including mosquito's infection, bee's venominfection, scorpion's venom infection, spider's venom infection,centipede's venom infection, ant's venom infection, and staphylinidae'svenom infection), reptile venom infection, a disease caused by abnormaleye cells (including cataract and retinopathy), a disease caused byabnormal brain cells (including Alzheimer's Disease and Parkinson'sdisease), and cell metabolism-related diseases (including pancreatitis,kidney inflammation, hepatitis, and cholangitis) occurred in vertebrate(preferably, mammalian).

In the present disclosure, when the pharmaceutical composition of thephenol red and the organic arsenic compound are used, the treatingefficacy of the combination is better than that of the organic arseniccompound alone, and the application field of the pharmaceuticalcomposition is also larger than that of the organic arsenic compoundalone. In addition, in the present disclosure, the experimental resultsindicate the pharmaceutical composition of the present disclosure hasgood treating effect on various diseases caused by different pathogens.The reason is that the active ingredients can combine with the receptorsof the glycoproteins in the pathogens, enter into the proteomic of thepathogens, and then restrict the localizations of the protein particles.Next, the morphology of the protein particles is changed, and theactivity thereof is loss. Hence, the pathogens cannot perform theirmetabolism and synthesis in the host cells, the polymerization of aminoacids for forming proteins is inhibited, and then the glycosylation ofthe pathogens is further inhibited, resulting in the structure of theprotein changed, the apoptosis of the proteins, and the synthesis,replication and differentiation of the pathogens inhibited. Therefore,the glycosylation pathway between the carbohydrates (such as sugar,starch and cellulose) and proteins, nucleoside and enzyme of thepathogens can be inhibited, and the diseases and disorders caused by thepathogens can be inhibited when the pharmaceutical composition of thepresent disclosure is used.

In addition, the phenol red in the pharmaceutical composition of thepresent disclosure can be used as a stabilizer of the organic arseniccompound (such as monosodium methanearsonate).

In the present disclosure, the phenol red and the organic arseniccompound can be combined into one formulation, or used in two separatedformulations.

In the present disclosure, the organic arsenic compound can be selectedfrom the group consisting of monosodium methanearsonate, methylarsonicacid, sodium dimethylarsonate, disodium methylarsonate, cacodylic acid,and calcium acid methanearsonate.

In the present disclosure, the phenol red has molecular weight of 354,and CAS No. thereof is 143-74-8. Monosodium methanearsonate hasmolecular weight of 162, and CAS No. thereof is 2163-80-6.

In the present disclosure, the glycoprotein-related disease can becaused by various viruses. Herein, the viruses classified by NationalCenter for Biotechnology Information, U.S. are listed below.

Family: Retroviridae

Subfamily: Orthoretrovirinae

-   -   Genus: Alpharetrovirus        -   species: Avian leukosis virus    -   Genus: Betaretrovirus        -   species: Jaagsiekte sheep retrovirus    -   Genus: Deltaretrovirus        -   species: Bovine leukemia virus        -   species: Primate T-lymphotropic virus 1        -   species: Primate T-lymphotropic virus 2        -   species: Primate T-lymphotropic virus 3    -   Genus: Gammaretrovirus        -   species: Feline leukemia virus        -   species: Gibbon ape leukemia virus (GALV)        -   species: Porcine type-C oncovirus    -   Genus: Lentivirus        -   species: Bovine immunodeficiency virus        -   species: Equine infectious anemia virus        -   species: Feline immunodeficiency virus        -   species: Human immunodeficiency virus 1        -   species: Human immunodeficiency virus 2        -   species: Puma lentivirus        -   species: Simian immunodeficiency virus        -   species: Visna/maedi virus

Subfamily: Spumaretrovirinae

-   -   Genus: Spumavirus        -   species: African green monkey simian foamy virus        -   species: Bovine foamy virus        -   species: Equine foamy virus        -   species: Feline foamy virus        -   species: Macaque simian foamy virus        -   species: Simian foamy virus            Family: Parvoviridae

Subfamily: Parvovirinae

-   -   Genus: Aveparvovirus        -   species: Chicken parvovirus        -   species: Chicken parvovirus ABU-P1        -   species: Gallus gallus enteric parvovirus        -   species: Turkey parvovirus 260    -   Genus: Bocaparvovirus        -   species: Pinniped bocaparvovirus 1        -   species: Pinniped bocaparvovirus 2        -   species: Primate bocaparvovirus 1        -   species: Primate bocaparvovirus 2        -   species: Ungulate bocaparvovirus 1        -   species: Ungulate bocaparvovirus 2        -   species: Ungulate bocaparvovirus 3        -   species: Ungulate bocaparvovirus 4        -   species: Ungulate bocaparvovirus 5    -   Genus: Copiparvovirus        -   species: Ungulate copiparvovirus 1        -   species: Ungulate copiparvovirus 2    -   Genus: Erythroparvovirus        -   species: Primate erythroparvovirus 1        -   species: Primate erythroparvovirus 2        -   species: Primate erythroparvovirus 3        -   species: Primate erythroparvovirus 4        -   species: Ungulate erythroparvovirus 1    -   Genus: Protoparvovirus        -   species: Canine parvovirus        -   species: Feline panleukopenia virus (strain 193)        -   species: Porcine parvovirus    -   Genus: Tetraparvovirus        -   species: Primate tetraparvovirus 1        -   species: Ungulate tetraparvovirus 1        -   species: Ungulate tetraparvovirus 2        -   species: Ungulate tetraparvovirus 3        -   species: Ungulate tetraparvovirus 4            Family: Paramyxoviridae

Subfamily: Paramyxovirinae

-   -   Genus: Avulavirus        -   species: Avian paramyxovirus 2        -   species: Avian paramyxovirus 3        -   species: Avian paramyxovirus 4        -   species: Avian paramyxovirus 5        -   species: Avian paramyxovirus 6        -   species: Avian paramyxovirus 7        -   species: Avian paramyxovirus 8        -   species: Avian paramyxovirus 9        -   species: Avian paramyxovirus 10        -   species: Avian paramyxovirus 11        -   species: Avian paramyxovirus 12        -   species: Newcastle disease virus    -   Genus: Henipavirus        -   species: Hendra virus        -   species: Nipah virus    -   Genus: Morbillivirus        -   species: Canine distemper virus        -   species: Measles virus        -   species: Rinderpest virus    -   Genus: Respirovirus        -   species: Bovine parainfluenza virus 3        -   species: Human parainfluenza virus 1        -   species: Human parainfluenza virus 3        -   species: Porcine parainfluenza virus 1        -   species: Sendai virus    -   Genus: Rubulavirus        -   species: Human parainfluenza virus 2        -   species: Human parainfluenza virus 4        -   species: Mapuera virus        -   species: Mumps virus        -   species: Parainfluenza virus 5        -   species: Porcine rubulavirus        -   species: Simian virus 41

Subfamily: Pneumovirinae

-   -   Genus: Metapneumovirus        -   species: Avian metapneumovirus        -   species: Human metapneumovirus    -   Genus: Orthopneumovirus        -   species: Bovine respiratory syncytial virus        -   species: Human respiratory syncytial virus        -   species: Murine pneumonia virus    -   Genus: Pneumovirus        -   species: Canine pneumovirus        -   species: Feline pneumovirus        -   species: Human respiratory syncytial virus A        -   species: Human respiratory syncytial virus B        -   species: Human respiratory syncytial virus S2        -   species: Human respiratory syncytial virus strain RSS-2        -   species: Human respiratory syncytial virus MinA        -   species: Human respiratory syncytial virus MinB        -   species: Human respiratory syncytial virus MinFLC        -   species: Human respiratory syncytial virus MinL        -   species: Ovine respiratory syncytial virus        -   species: Pneumovirus dog/Ane4/USA/2008        -   species: Pneumovirus dog/Bme17/USA/2008        -   species: Pneumovirus HFR-2013        -   species: Respiratory syncytial virus type A            Family: Coronaviridae

Subfamily: Coronavirinae

-   -   Genus: Alphacoronavirus        -   species: Canine coronavirus        -   species: Feline coronavirus        -   species: Human coronavirus 229E        -   species: Human coronavirus NL63        -   species: Porcine epidemic diarrhea virus        -   species: Transmissible gastroenteritis virus    -   Genus: Betacoronavirus        -   species: Bovine coronavirus        -   species: Canine respiratory coronavirus        -   species: Equine coronavirus        -   species: Human coronavirus OC43        -   species: Porcine hemagglutinating encephalomyelitis virus        -   species: Human coronavirus HKU1        -   species: Human coronavirus type 5        -   species: Middle East respiratory syndrome coronavirus        -   species: Severe acute respiratory syndrome-related            coronavirus    -   Genus: Deltacoronavirus        -   species: Porcine coronavirus HKU15    -   Genus: Gammacoronavirus        -   species: Duck coronavirus        -   species: European turkey coronavirus 080385d        -   species: Goose coronavirus        -   species: Infectious bronchitis virus        -   species: Pheasant coronavirus        -   species: Pigeon coronavirus        -   species: Turkey coronavirus

Subfamily: Torovirinae

-   -   Genus: Torovirus        -   species: Bovine torovirus        -   species: Equine torovirus        -   species: Human torovirus        -   species: Porcine torovirus            Family: Herpesviridae

Subfamily: Alphaherpesvirinae

-   -   Genus: Iltoviru        -   species: Gallid herpesvirus 1        -   species: Psittacid herpesvirus 1    -   Genus: Mardivirus        -   species: Anatid herpesvirus 1        -   species: Columbid herpesvirus 1        -   species: Gallid herpesvirus 2        -   species: Gallid herpesvirus 3        -   species: Meleagrid herpesvirus 1 (Turkey herpesvirus)    -   Genus: Simplexvirus        -   species: Bovine herpesvirus 2        -   species: Human herpesvirus 1 (Herpes simplex virus type 1)        -   species: Human herpesvirus 2        -   species: Macacine herpesvirus 1 (monkey B virus)    -   Genus: Varicellovirus        -   species: Bovine herpesvirus 1        -   species: Bovine herpesvirus 5        -   species: Canid herpesvirus 1        -   species: Caprine herpesvirus 1 (goat herpesvirus)        -   species: Equid herpesvirus 1 (Equine herpesvirus 1)        -   species: Equid herpesvirus 3        -   species: Equid herpesvirus 4 (Equine herpesvirus 4)        -   species: Equid herpesvirus 8        -   species: Equid herpesvirus 9        -   species: Felid herpesvirus 1        -   species: Human herpesvirus 3 (Varicella-zoster virus)        -   species: Suid herpesvirus 1 (Pseudorabies virus)

Subfamily: Betaherpesvirinae

-   -   Genus: Cytomegalovirus        -   species: Aotine herpesvirus 1        -   species: Cebine herpesvirus 1        -   species: Human herpesvirus 5 (Human cytomegalovirus)        -   species: Macacine herpesvirus 3 (Rhesus cytomegalovirus)        -   species: Panine herpesvirus 2 (Chimpanzee cytomegalovirus)    -   Genus: Proboscivirus        -   species: Elephantid herpesvirus 1    -   Genus: Roseolovirus        -   species: Human herpesvirus 6A        -   species: Human herpesvirus 6B        -   species: Human herpesvirus 7

Subfamily: Gammaherpesvirinae

-   -   Genus: Lymphocryptovirus        -   species: Human herpesvirus 4 (Epstein-Barr virus)    -   Genus: Macavirus        -   species: Bovine herpesvirus 6        -   species: Caprine herpesvirus 2        -   species: Ovine herpesvirus 2    -   Genus: Percavirus        -   species: Equid herpesvirus 2 (Equine herpesvirus 2)        -   species: Equid herpesvirus 5    -   Genus: Rhadinovirus        -   species: Bovine herpesvirus 4        -   species: Human herpesvirus 8

In the present disclosure, a pharmaceutical acceptable carrier can befurther administered to the subject in need thereof. Hence, thepharmaceutical composition of the present disclosure may furthercomprises the pharmaceutical acceptable carrier. Herein, thepharmaceutical acceptable carrier is selected from the group consistingof solvent, buffer (such as phosphate buffered saline (PBS), Ringer'ssolution and Hank's solution), suspending agent, decomposer,disintegrating agent, dispersing agent, binding agent, excipient,stabilizing agent, chelating agent, diluent, gelling agent,preservative, lubricant, absorption delaying agent, and liposome.

In the present disclosure, the pharmaceutical composition can beformulated into any formulation known in the art. The formulationscomprise, but are not limited to: injection (for example, sterileaqueous solution or dispersion), sterile powder, tablet, troche, patch,lozenge, capsule, dispersible powder or granule, solution, suspension,emulsion, syrup, elixir, slurry and the like.

In the present disclosure, the term “treating” or refers toadministering the phenol red and the organic arsenic compound to asubject, who has an above-described disease, a symptom of such adisease, or a predisposition toward such a disease, with the purpose toconfer a therapeutic effect, e.g., to cure, relieve, alter, affect,ameliorate, or prevent the above-described disease, the symptom of it,or the predisposition toward it.

In the present disclosure, the term “a first or second effective amount”refers to the amount of the phenol red or the organic arsenic compoundthat is required to confer a therapeutic effect on the treated subject.Effective amounts may vary, as recognized by those skilled in the art,depending on the types of diseases treated, route of administration,excipient usage, and the possibility of co-usage with other agents.

In the present disclosure, the phenol red and the organic arseniccompound can be administered via any known rout known in the art, forexample, parenteral routes, intraperitoneal injection, subcutaneousinjection, intramuscular injection and intravenous injection.Preferably, the phenol red and the organic arsenic compound areadministered via intramuscular injection or intravenous injection.

In the present disclosure, the dose and the administering times of thephenol red and the organic arsenic compound can be varied based on thefollowing factors: the severity of the disease to be treated, theadministering rout, and the age, condition and reaction of the subjectto be treated. Generally, the dose of the phenol red and the organicarsenic compound is administered based on the bodyweight of the subject,and can be administered in a single dosage or several dosages, as longas the phenol red and the organic arsenic compound are administered atthe same time or within several minutes.

In the present disclosure, the first effective amount of the phenol redis not particularly limited, and can be adjusted according to theseverity of the disease. Preferably, the first effective amount isranged from 0.1 mg to 5.0 mg per kilograms of the subject in needthereof. More preferably, the first effective amount is ranged from 0.5mg to 1.5 mg per kilograms of the subject in need thereof.

In the present disclosure, the second effective amount of the organicarsenic compound is also not particularly limited, and can be adjustedaccording to the severity of the disease. Preferably, the secondeffective amount is ranged from 0.1 mg to 10.0 mg per kilograms of thesubject in need thereof. More preferably, the second effective amount isranged from 0.8 mg to 2.0 mg per kilograms of the subject in needthereof.

In the present disclosure, when the phenol red is formulated into asolution, the first effective amount is ranged from 0.1 mg/cc to 5.0mg/cc, and preferably from 0.8 mg/cc to 3.0 mg/cc.

In addition, in the present disclosure, when the organic arseniccompound is formulated into a solution, the second effective amount isranged from 0.1 mg/cc to 10.0 mg/cc, and preferably from 2.0 mg/cc to6.0 mg/cc.

Other objects, advantages, and novel features of the invention willbecome more apparent from the following detailed description when takenin conjunction with the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a FIGURE showing the leukocyte amount changes of the FIVinfected cat during the treatment according to Embodiment 1 of thepresent disclosure.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

The present invention has been described in an illustrative manner, andit is to be understood that the terminology used is intended to be inthe nature of description rather than of limitation. Many modificationsand variations of the present invention are possible in light of theabove teachings. Therefore, it is to be understood that within the scopeof the appended claims, the invention may be practiced otherwise than asspecifically described.

In the following embodiments, a solution containing phenol red (2 mg/cc)and monosodium methanearsonate (4 mg/cc) is used, which is named as“LuMC5”, hereinafter. In addition, a solution containing monosodiummethanearsonate (4 mg/cc) alone is also used, which is named as “MASA”,hereinafter. It should be noted that the following embodiments onlyprovide one formulation of the pharmaceutical composition of the presentdisclosure. A person skilled in the art knows that the concentration ofthe active ingredients and the contents of the pharmaceuticalcomposition can be adjusted within the scope of the appended claims.

Embodiment 1

In the present embodiment, feline immunodeficiency virus (FIV) which isbelonged to the same family (Retroviridae) and the same genus(Lentivirus) to HIV, was selected to perform the present clinicalexperiment. LuMC5 was administered to the infected cat via intravenousinjection or intramuscular injection, 0.4 cc/kg, once or twice a day.The results are shown in the following Table 1 and FIG. 1.

TABLE 1 Blood cells examination data of FIV infected cat during thetreatment (Testing company: LEZEN Reference Lab) WBC NEU LYM MONO EOSIBASO Day 1 530 18.9 43.4 37.7 0 0 Day 3 12790 73 10 6.6 0.3 0.1 Day 931670 80 9 6 4.9 0.1 Day 34 9000 69 24 5 2 0.1 RBC Hgb HCT M.C.V M.C.HM.C.H.C PLT Day 1 7.84 12.1 44.5 56.8 15.4 27.2 56 Day 3 7.7 11.9 42.154.7 15.5 28.3 165 Day 9 6.82 10.6 34.8 51 15.5 30.5 132 Day 34 6.7110.2 37.5 55.9 15.2 27.2 38 NEU: Neutrophils/LYM: Lymphocytes/MONO:Monocytes/EOSI: Eosinophils/BASO: Basophils/Hg: Hemoglobin/HCT:Hematocrit/

From the results shown in FIG. 1 and Table 1, the immune cell amount wasincreased rapidly at Day 3. In addition, acquired immune deficiencysyndromes caused by FIV, such as toxoplasmosis, pneumocystis pneumoniaand tumors were not found. The FIV infected cat turned into a healthycarrier after treatment.

In addition, LuMC5 can be administered to the FIV infected cat for 4 to14 days. No virus is detected after 4 to 14 days. Hence, LuMC5 can beadministered when the virus levels in the FIV infected cat is high tocontrol the syndromes, and is not have to be administered for a longterm. Therefore, the immune system of the FIV infected cat is notdestroyed.

Therefore, when LuMC5 is used for treating the FIV infected subject, thedevelopment of the acquired immune deficiency syndromes caused by FIVcan be inhibited; and the complication such as toxoplasmosis andpneumocystis pneumonia can be inhibited. Hence, the purposes oftreatment and prevention can be accomplished.

Embodiment 2

In the present embodiment, MASA and LuMC5 were used to treat the virusinfected vertebrate and mammalian. 7 virus species belonged to 5families were selected to perform the present clinical experiment.Herein, the virus classification is on the basis of National Center forBiotechnology Information, U. S.

Subject: Infected dogs and cates sent to Hei-Ming Veterinary Hospital,Taiwan

7 virus species for clinical experiments:

Family: Retroviridae

-   -   Genus: Gammaretrovirus        -   species: Feline leukemia virus    -   Genus: Lentivirus        -   species: Feline immunodeficiency virus            Family: Parvoviridae    -   species: Canine parvovirus    -   species: Feline panleukopenia virus        Family: Paramyxoviridae    -   Genus: Morbillivirus        -   species: Canine distemper virus            Family: Coronaviridae    -   Genus: Alphacoronavirus        -   species: Feline coronavirus            Family: Herpesviridae    -   Genus: Varicellovirus        -   species: Felid herpesvirus 1            Treatment: Intravenous injection or intramuscular injection,            0.4 cc/kg, once or twice a day            Testing company: Genomics BioSci & TechCo., Ltd.            (Abbreviation: G)    -   MountainVet Biotech Co., Ltd. (Abbreviation: M)    -   VETE Co., Ltd. (Abbreviation: V)    -   LEZEN Reference Lab (Abbreviation: L)        Testing instrument: Real-time quantitative PCR detecting system        (sensitivity>99%) for G and M companies; and PCR or RT-PCR for V        company        Testing method: The detected genes are the long terminal repeat        (LTR) gene of Feline immunodeficiency virus, the virus protein 2        (VP2) gene of Canine parvovirus and the nucleocapsid gene (N        gene) of Canine distemper virus. The virus amount is a curve        calculated by the cycle threshold (C_(t)) values of the obtained        PCR products based on the cycle threshold (C_(t)) values of the        standard with predetermined different concentrations.

The testing results of the aforementioned 7 viruses in the clinicalexperiments are listed in the following Tables 2-1 to 2-7.

TABLE 2-1 Results of the clinical experiments on Feline leukemia virusAnimal Total Detecting Results & ID Age Gender Medicine Days point(ng/uL) Specimen Company Cat 2 M MSMA 9 Day 0 + (*^(a)) Blood V 130730Years Day 9 − (*^(b)) Cat 7 F LuMC5 9 Day 0 222.8 × 10⁻⁷ Blood M 160312Years  Day 11 98.75 × 10⁻⁷ Cat 1 F LuMC5 7 Day 0 248.8 × 10⁻⁷ Blood M150903 Years Day 9 87.51 × 10⁻⁷ Cat 2 F LuMC5 6 Day 0 261.4 × 10⁻⁷ BloodM 160729 Months Day 4  74.6 × 10⁻⁷ Day 6  53.6 × 10⁻⁷

TABLE 2-2 Results of the clinical experiments on feline immunodeficiencyvirus Animal Total Detecting Results & ID Age Gender Medicine Days point(ng/uL) Specimen Company Cat 2 M MSMA 9 Day 0 + (*^(a)) Blood V 130730Years Day 9 − (*^(b)) Cat 7 F MSMA 14 Day 0 + (*^(a)) Blood V 130805Months  Day 14 − (*^(b)) Cat 7 F LuMC5 9 Day 0 45.75 × 10⁻⁷ Blood M160312 Years  Day 11 • Cat 2 F LuMC5 6 Day 0  34.8 × 10⁻⁷ Blood M 160729Months Day 4 • Day 6 •

TABLE 2-3 Results of the clinical experiments on canine parvovirusAnimal Total Detecting Results & ID Age Gender Medicine Days point(ng/uL) Specimen Company Dog 6 F MSMA 4 Day 0 932918 × 10⁻⁸   Feces (*c)G 140529 Months Day 4 28836 × 10⁻⁸  Dog 3 F MSMA 10 Day 0 458306 ×10⁻⁸   Blood M 150716 Months  Day 10 2470 × 10⁻⁸  Feces (*d) Dog 2 FMSMA 10 Day 0 40724.24 × 10⁻⁷   Blood M 150708 Months Day 8 2696.9 ×10⁻⁷   Day 15 185.6 × 10⁻⁷  Dog 1 M MSMA 7 Day 0 46322 × 10⁻⁸  Blood G150125 year Day 3  345 × 10⁻⁸ Day 5  75 × 10⁻⁸ Day 6  40 × 10⁻⁸  Day 11• Dog 5 F MSMA 10 Day 0 105804 × 10⁻⁸   Blood G 140413 Months Day 3 3560× 10⁻⁸  Day 9 5314 × 10⁻⁸   Day 11 183.7 × 10⁻⁸  Dog 2 M LuMC5 3 Day 036630.6 × 10⁻⁷   Blood M 151123 Months Day 3 1111.5 × 10⁻⁷  Dog 3 MLuMC5 5 Day 0 102094.9 × 10⁻⁷   Blood M 160301 Months Day 5 66.9 × 10⁻⁷Dog 5 M LuMC5 4 Day 0 62749.9 × 10⁻⁷   Blood M 160308 Months Day 4 7.61× 10⁻⁷ Dog 3 M LuMC5 4 Day 0 6.66 × 10⁻⁷ Blood M 160311 Months Day 4 •Dog 5 M LuMC5 3 Day 0 6474.4 × 10⁻⁷  Blood M 160324 Years Day 3 87.7 ×10⁻⁷ Dog 8 F LuMC5 4 Day 0 107633.3 × 10⁻⁷   Blood M 160326 Months Day 377.4 × 10⁻⁷

TABLE 2-4 Results of the clinical experiments on feline panleukopeniavirus Animal Total Detecting Results & ID Age Gender Medicine Days point(ng/uL) Specimen Company Cat 3 F MSMA 12 Day 0 4240717 × 10⁻⁸   Feces(*c_) G 150323 Months Day 6 22391915 × 10⁻⁸      Day 15  711 × 10⁻⁸  Day21   14 × 10⁻⁸ Cat 3 M MSMA 10 Day 0 130942 × 10⁻⁸  Blood G 141128Months Day 6  1088 × 10⁻⁸ Day 7   25 × 10⁻⁸ Day 8   79 × 10⁻⁸ Cat 3 FMSMA 8 Day 0 143.67 × 10⁻⁷  Blood G 140512 Years Day 7 • Cat 3 F MSMA 12Day 0 121.7 × 10⁻⁷ Blood G 150529 Years  Day 15 • Cat 7 M LuMC5 7 Day 011.21 × 10⁻⁷ Feces (*c) M 150826 Months Day 5  2.47 × 10⁻⁷ Cat 8 M LuMC58 Day 0 40784.1 × 10⁻⁷  Blood M 160104 Months Day 8 10.28 × 10⁻⁷ Cat 8 FLuMC5 7 Day 0 4219.6 × 10⁻⁷  Blood M 160108 Months Day 7 78.91 × 10⁻⁷

TABLE 2-5 Results of the clinical experiments on canine distemper virusAnimal Total Detecting Results & ID Age Gender Medicine Days point(ng/uL) Specimen Company Dog 4 F MSMA 14 Day 0 1341 × 10⁻⁸  Nasal G140701 Months Day 4 334 × 10⁻⁸ secretion (*c)  Day 10 116 × 10⁻⁸  Day 14• Dog 4 F MSMA 12 Day 0  24 × 10⁻⁸ Nasal G 140703 Months Day 3 312 ×10⁻⁸ secretion (*c)  Day 10  61 × 10⁻⁸  Day 12 • Dog 1 F MSMA 11 Day 0310 × 10⁻⁸ Blood G 140808 Year Day 7 350 × 10⁻⁸  Day 11 • Dog 7 F MSMA12 Day 0 350 × 10⁻⁸ Blood G 140808-1 Years Day 8 264 × 10⁻⁸  Day 12 •Dog 12 M MSMA 3 Day 0  28 × 10⁻⁸ Blood G 140617 Years Day 3 •

TABLE 2-6 Results of the clinical experiments on feline coronavirusAnimal Total Detecting Results & ID Age Gender Medicine Days point(ng/uL) Specimen Company Cat 5 F MSMA 8 Day 0 41733.8 × 10⁻⁷ Blood G150430 Years Day 8 • Cat 4 M LuMC5 8 Day 0 5691.51 × 10⁻⁷ Feces (*c) M150826 Months Day 5  176.05 × 10⁻⁷ Day 8 • Cat 5 M LuMC5 5 Day 010589.77 × 10⁻⁷ Blood M 150903 Months Day 5 • Cat 7 F LuMC5 8 Day 0 125.8 × 10⁻⁷ Blood M 160110 Months Day 8 • Ascites

TABLE 2-7 Results of the clinical experiments on felid herpesvirus 1Animal Total Detecting Results & ID Age Gender Medicine Days point(ng/uL) Specimen Company Cat 1 M MSMA 12 Day 0 1528214 × 10⁻⁸   Blood G150417 Year Day 4  214 × 10⁻⁸  Day 13 • Cat 5 M MSMA 10 Day 0 151.8 ×10⁻⁷ Nasal G 150502 Months  Day 10  3.75 × 10⁻⁷ secretion (*c) Cat 3 FMSMA 9 Day 0 27380 × 10⁻⁷  Blood G 150507 years Day 7 • Cat 8 F MSMA 8Day 0 2282.5 × 10⁻⁷  Blood G 150516 Months  Day 15 69.71 × 10⁻⁷ Cat 8 FLuMC5 5 Day 0 326.1 × 10⁻⁷ Blood M 151224 Months Day 5 • Cat 11 F LuMC56 Day 0 8911.7 × 10⁻⁷  Blood M 160315 Months Day 6 661.6 × 10⁻⁷ (*a) ”+”refers to viruses were found in the detection. (*b) ”−” refers toviruses were not found in the detection. (*c) Not quantitative detection• No pathogen infection or small amount of pathogens detected

The administered period of MASA was 4 days to 14 days (average 9 days).The administered period of LuMC5 was 3 days to 9 days (average 5.7days). When LuMC5 was administered via subcutaneous injection,intramuscular injection or intravenous injection, the fever in thesubject was reduced within 3 hours to 5 hours, and metal condition ofthe subject was improved. The virus level in the specimens thereof wasgreatly reduced to slight amount within 2 days to 5 days, and reducedfrom the slight amount to the amount incapable to be detected within 5days to 8 days. The infection syndromes were rapidly improved.

Embodiment 3

In the present embodiment, LuMC5 was used to treat the mammalian withhemorrhagic venom infection and neurotoxic venom.

Subject: Infected dogs and cates sent to Hei-Ming Veterinary Hospital,Taiwan

Treatment: Intravenous injection or intramuscular injection, 0.4 cc/kg,once or twice a day

The testing results s in the clinical experiments of the presentembodiment are listed in the following Tables 3-1 to 3-3.

TABLE 3-1 Results of the clinical experiments on the subject infectedwith hemorrhagic venom infection of Trimeresurus mucrosquamatus AnimalTime to Administered Syndromes before treatment & ID Age Gender betreated times Condition after treatment Dog 3 M 2 hours 1 Depression,lifeless, sticky saliva, and 130420 years after facial and head swellinginfection Depression and lifeless were relieved, 1 hours aftertreatment. Facial and head swelling were relieved, 12 hours aftertreatment. Dog 6 F 2 hours 2 Facial swelling, depression, lifeless, and140405 years after mouth and tongue blood stasis infection Depressionand lifeless were relieved, 30 minutes after treatment. Facial swellingand mouth and tongue blood stasis were relieved, 24 hours aftertreatment. Cat 1 F 1 hours 2 Shock, unconsciousness, tachypnoea, 140320year after transparent and jelly-like saliva, and infection hematuriaShock, unconsciousness, tachypnoea, and transparent and jelly-likesaliva were relieved, 30 minutes after treatment. Hematuria was relievedand the subject became healthy, 50 minutes after treatment. Dog 3 M 26hours 2 Depression, lifeless, sticky saliva, and chin 140724 years afterswelling infection Depression, lifeless, and sticky saliva wererelieved, 50 minutes after treatment. Chin swelling was relieved, 12hours after treatment. Dog 4 M 4 hours 1 Bleed and depression 140707years after Bleed caused by snakebite was relieved, 10 infection minutesafter treatment. Depression was relieved, 30 minutes after treatment.Dog 4 M 4 hours 1 Bleed and depression 140707-1 years after Bleed causedby snakebite was relieved, 10 infection minutes after treatment.Depression was relieved, 30 minutes after treatment. Dog 7 M 16 hours 2Depression, lifeless, sticky saliva, and 141028 years after facial andchin swelling infection Depression and lifeless were relieved, 30minutes after treatment. Facial and chin swelling was relieved, 12 hoursafter treatment.

TABLE 3-2 Results of the clinical experiments on the subject infectedwith hemorrhagic venom infection of Viridovipera stejnegeri SyndromesAdmin- before treatment Animal Time to istered Condition & ID Age Genderbe treated times after treatment Dog 1 M 14 hours 3 Depression,lifeless, 140412 year after sticky saliva, and infection facial and chinswelling Depression and lifeless were relieved, 2 hours after treatment.Facial and chin swelling was relieved, 24 hours after treatment.

TABLE 3-3 Results of the clinical experiments on the subject infectedwith neurotoxic venom infection of Naja Syndromes Admin- beforetreatment Animal Time to istered Condition & ID Age Gender be treatedtimes after treatment Dog 6 M 2 hours 2 Depression, lifeless, 140426years after sticky saliva, and infection facial and chin swellingDepression and lifeless were relieved, 30 minutes after treatment.Facial and chin swelling was relieved, 12 hours after treatment.

From the results shown in the present embodiment, when LuMC5 wasadministered into the infected subject via subcutaneous injection,intramuscular injection or intravenous injection, the nose bleeding andsticky saliva can be relieved within 10 minutes, the depression can berelieved within 30 minutes, the hematuria can be relieved within 50minutes, and the swelling can be relieved and the treated subject becomehealthy within 1 hours to 36 hours. Hence, by using LuMC5 of the presentdisclosure, the infection syndromes can be controlled. Compared to theconventional method by naturalizing toxins with serum formulation, inwhich antibodies are formed to identify and neutralize toxin proteinsafter 7 days to 14 days and the swelling syndrome is relieved after 7days, LuMC5 of the present disclosure can relieve the syndromes in ashort time and has superior treatment efficacy.

Embodiment 4

In the present embodiment, LuMC5 was used to treat the mammalian thatthe mosquito, bee, scorpion, spider, centipede, ant, and staphylinidaebitten.

Subject: Dog bit by bees, sent to Hei-Ming Veterinary Hospital, Taiwan,and having bodyweight of 27 Kg

Treatment: Intravenous injection or intramuscular injection, 0.4 cc/kg,twice a day

The result is shown in the following Table 4-1.

TABLE 4-1 Syndromes Admin- before treatment Animal Time to isteredCondition & ID Age Gender be treated times after treatment Dog 14 F 2hours 2 Depression, lifeless, 140414 years after and facial and headinfection swelling Depression and lifeless were relieved, 30 minutesafter treatment Facial and head swelling was relieved, 12 hours aftertreatment.

The treatment results show that the mental condition of the treatedsubject was improved, the appetite thereof was recovered, and theactivity thereof such as tail shaking turned into normal, aftertreatment.

Subject: Human bit by a mosquito, bodyweight: 79 Kg

Treatment: Applying LuMC5 at the mosquito biting region, twice a day

The result is shown in the following Table 4-2.

TABLE 4-2 Syndromes Admin- before treatment Animal Time to isteredCondition & ID Age Gender be treated times after treatment Dog 55 F 3minutes 2 Swelling and itch at the 160415 years after biting region onthe skin infection Itch was relieved, 3-20 minutes after applyingmedicine. Swelling was relieved, 1-3 hours after applying medicine.

From the results shown in the present embodiment, LuMC5 can effectivelyrelieve the itch and selling on the skin caused by the insects. If thesubject is bit by the insects, the glycosylation of the virus can beinhibited when LuMC5 is applied immediately. In addition, the syndromesof venom allergy, fever and lifeless can be relieved by administeringLuMC5 via injection. The swelling at the skin region bit by the insectswith pathogens can be relieved by applying ointment or solution at theaffected region. Therefore, Dengue virus, West Nile virus and Zika virusinfection to human and animals can be effectively relieved and preventedby using LuMC5 of the present disclosure.

Embodiment 5

In the present embodiment, LuMC5 was used to treat cataract andretinopathy caused by abnormal eye cells in mammalian.

Subject: Infected dogs and cates sent to Hei-Ming Veterinary Hospital,Taiwan

Treatment: Solution containing 1-2 mg/cc of phenol red and 3-4 mg/cc ofmonosodium methanearsonate, one drop per time, twice or three times aday

The result is shown in the following Tables 5-1 and 5-2.

TABLE 5-1 Results of the clinical experiments on Pomeranian withcataract Syndromes before treatment Animal Total Condition & ID AgeGender days Treatment after treatment Dog 18 F 46 One drop Maturecataract, spin 151013 years per time, around while walking, three timesa incapable of walking day straight, hitting the wall, and recognizingsurrounding by smelling, auditory sense and living experience Theclouding of the lens in the eye reduced. The treated subject can walkstraight without hitting the wall.

TABLE 5-2 Results of the clinical experiments on Golden Retriever withcataract Syndromes before treatment Animal Total Condition & ID AgeGender days Treatment after treatment Dog 10 M 29 One drop The subjectcannot see 160128 years per time, the object at the long twice a daydistance during daytime. Visually impaired during the night. Theclouding of the lens in the eye reduced. The subject's day and nightvision was recovered.

Embodiment 6

In the present embodiment, LuMC5 was used to treat metabolism-relateddiseases (including pancreatitis, kidney inflammation, hepatitis, andcholangitis) occurred in mammalian.

Subject: Infected dogs and cates sent to Hei-Ming Veterinary Hospital,Taiwan

Treatment: Intravenous injection or intramuscular injection, 0.4 cc/kg,once a day

Testing company: LEZEN Reference Lab

The result is shown in the following Tables 6-1 to 6-4.

TABLE 6-1 Syndromes before treatment Animal Total Condition & ID AgeGender days Body weight after treatment Dog 15 M 11 20 Kg Anorexia for4-5 160714 years days, urine in dark brown, weakness, and dehydrationAppetite and activity recovered

TABLE 6-2 Blood test data of Dog 160714 during the treatment Alkaline-PT:ALT p BUN Creatinine Amylase (liver) (gall) (uremia) (Kidney)(pancreas) Day 1 87 87 94.2 3.44 1447 Day 19 24 50 60 2.78 1225Reference 14-40 U/L 32-91 U/L 8-20 0.64-1.27 S: 28-1200 mg/dL mg/dL U/L

TABLE 6-3 Syndromes before treatment Animal Total Condition & ID AgeGender days Body weight after treatment Dog 15 F 3 28 Kg Anorexia for1-2 days, 160805 years vomit, weakness, incapable of standing Capable ofstanding at Day 2, eating small amount of food, and walking slowly atDay 3

TABLE 6-4 Blood test data of Dog 160805 during the treatment CreatinineAmylase Item BUN (uremia) (Kidney) (pancreas) 2016 Aug. 5 31.5 2.46 12512016 Aug. 8 21.5 1.19 931 Reference 8-20 mg/dL 0.64-1.27 mg/dL S:28-1200 U/L

From the results shown in Embodiments 5 and 6, a solution made fromLuMC5 can be used to treat diseases caused by abnormal eye cells (suchas cataract and retinopathy) by administering the solution at thepredetermined time with a predetermined amount. When treating diseasescaused by abnormal brain cells (including Alzheimer's Disease andParkinson's disease), LuMC5 can be formulated into a paste or ointmentand administered at the predetermined time with a predetermined amount.The skin absorbs the active ingredients, and the active ingredients canenter into the brain via the blood in the carotid artery to accomplishthe purpose of preventing disease. When treating metabolism relateddiseases (such as pancreatitis, kidney inflammation, hepatitis, ascholangitis), LuMC5 can be administered via subcutaneous injection,intramuscular injection or intravenous injection for 5 to 7 days, andthe swelling and inflammation can be effectively relieved.

Embodiment 7

When treating prion infection (including bovine spongiformencephalopathy, scrapie, and creutzfeldt-Jakob disease), LuMC5 can beformulated into a paste or ointment and administered at thepredetermined time with a predetermined amount. The skin absorbs theactive ingredients, and the active ingredients can enter into the brainvia the blood in the carotid artery to accomplish the purpose ofpreventing disease.

Although the present invention has been explained in relation to itspreferred embodiment, it is to be understood that many other possiblemodifications and variations can be made without departing from thespirit and scope of the invention as hereinafter claimed.

What is claimed is:
 1. A method for treating a glycoprotein-relateddisease, comprising: administering a first effective amount of phenolred and a second effective amount of an organic arsenic compound to amammal in need thereof; wherein the glycoprotein-related disease iscaused by one virus selected from the group consisting of: felineleukemia virus, feline immunodeficiency virus, canine parvovirus, felinepanleukopenia virus, feline coronavirus and feline herpesvirus 1; andwherein the organic arsenic compound is monosodium methanearsonate. 2.The method of claim 1, wherein a pharmaceutical acceptable carrier isfurther administered to the mammal in need thereof.
 3. The method ofclaim 2, wherein the pharmaceutical acceptable carrier is selected fromthe group consisting of solvent, buffer, suspending agent, decomposer,disintegrating agent, dispersing agent, binding agent, excipient,stabilizing agent, chelating agent, diluent, gelling agent,preservative, lubricant, absorption delaying agent, and liposome.
 4. Themethod of claim 1, wherein the first effective amount is ranged from 0.1mg to 5.0 mg per kilograms of the mammal in need thereof.
 5. The methodof claim 4, wherein the first effective amount is ranged from 0.5 mg to1.5 mg per kilograms of the mammal in need thereof.
 6. The method ofclaim 1, wherein the first effective amount is ranged from 0.1 mg/cc to5.0 mg/cc.
 7. The method of claim 1, wherein the first effective amountis ranged from 0.8 mg/cc to 3.0 mg/cc.
 8. The method of claim 1, whereinthe second effective amount is ranged from 0.1 mg to 10.0 mg perkilograms of the mammal in need thereof.
 9. The method of claim 8,wherein the second effective amount is ranged from 0.8 mg to 2.0 mg perkilograms of the mammal in need thereof.
 10. The method of claim 1,wherein the second effective amount is ranged from 0.1 mg/cc to 10.0mg/cc.
 11. The method of claim 10, wherein the second effective amountis ranged from 2.0 mg/cc to 6.0 mg/cc.